Embryo Monitoring and Cultivation

Embryo time-lapse monitoring is an advanced technique used in assisted reproductive treatments to closely observe the development of embryos. The principle of this method is to capture continuous images of the embryos as they develop, providing detailed information on their growth patterns and improving the selection of the best embryos for transfer.

This method is particularly suitable in the following cases:

• When couples have experienced previous IVF failures

• When there are multiple embryos and the best ones need to be selected
• When detailed monitoring of embryo development is required
• In other cases, depending on the physician's recommendation

How does Embryo Time-Lapse Monitoring work?

1. Continuous Imaging: The embryos are placed in a special incubator equipped with a built-in camera that takes images at regular intervals, capturing their development from fertilization to the blastocyst stage.
2. Detailed Analysis: These images are compiled into a time-lapse video, allowing embryologists to analyse the growth patterns and developmental milestones of each embryo.
3. Selection of Embryos: Based on a detailed analysis, the most viable embryos are selected for transfer, improving the chances of successful implantation and pregnancy.

Embryo time-lapse monitoring provides valuable insights into embryo development, helping to optimize the selection process and to enhance the likelihood of a successful outcome.

The principle of this method is to culture embryos for a longer period, usually until the blastocyst stage (5-6 days), to allow for better assessment of their development and viability.

Typically, embryos are cultured under laboratory conditions for 5 days, during which they divide into 4-8 cells, until the blastocyst stage. Extending the culture period to 5-6 days increases the chances of selecting the highest-quality embryos for transfer or freezing. 

How does Extended Cultivation work?

1. Embryo Culturing: After fertilization, embryos are cultured in a specialized incubator for an extended period, typically 5 to 6 days, until they reach the blastocyst stage.
2. Monitoring Development: During this time, embryologists closely monitor the development of the embryos, assessing factors such as cell division, growth rate, and overall morphology.
3. Selection of Embryos: The most viable blastocysts are selected for transfer or freezing, as they have a higher chance of successful implantation and pregnancy.

Like any lab technique, prolonged embryo culture has its advantages and disadvantages. By extending the culture period and monitoring, we can better identify embryos with the highest potential. On average, 30-40% of embryos reach the blastocyst stage, indicating a robust selection process. Extended cultivation allows for a more accurate assessment of embryo quality, helping to optimize the selection process and to increase the likelihood of a successful outcome.